Equine Infectious Anemia is an equine infectious disease caused by a lentivirus, the Equine Infectious Anemia virus. The disease is characterized by three different clinical forms: acute, chronic and inapparent.
The lentivirus has RNA as genetic material. Despite of the genetic mutations between the different virus samples, the p26 protein used in diagnostic tests is stable and specific to all EIA virus samples.
The EIA virus shows in vivo macrophage tropism, but in vitro it can replicate in other cells. Viral replication is performed in long-term fibroblastic cell cultures arising from 0.2 to 1.2 µm vesicles originated from the equine dermis (photograph of the cell below).
The immunodiffusion technique in agar is the the "golden diagnostic test" for EIA. The test detects antibodies against the p26 antigen from the EIA virus (main protein of the viral core).
The advantage that the immunodiffusion technique has in the diagnostic of the Equine Infectious Anemia is the ability to differentiate antigens that are molecularly close due to the identity reaction.
Create your own web pages in minutes...
Copyright 1989-2007 Laboratórios Bruch Ltda.
p26 Antigen of the Equine Infectious Anemia Virus
Cell with 20 µm in diameter. Double nuclear membrane is distinguished in the bottom edge of the nucleus (big arrows). Presence of nuclear pores (small arrows). In the cytoplasm and around the nucleous, there are several Golgi formations, two of them with fused cisternae (G). Secretory vesicles with granulations inside (vd) with average size of 400 nm.
Picture: Laboratórios Bruch Ltda, registred in INPI, required patent.
NEWS
Certified for NBR ISO/IEC 17025:2005
PRODUCTS